![]() ![]() The Bart lab will perform isolations following their established protocols (Bart et al., 2012). ![]() Rebecca Bart at the Donald Danforth Plant Science Center. Bart.Characterize the T3Es of a diverse collection of Xcm isolates (Bart): Leaf samples from cotton fields showing evidence of cotton bacterial blight infection will be sent to Dr. He will stay on top of the overall disease situation, and encourage specialists in other states outside of those listed on this project to also submit disease samples to Dr. Woodward will coordinate with the extension specialists in other states, including Mississippi and Georgia, but not excluding other states that may develop bacterial blight on their cotton. Samples will be refrigerated after collection until they are sent to Dr. Information collected to accompany samples will include date, location (for geo-referencing), cotton cultivar and general level of disease severity. Sample units will consist of individual infected leaves, bolls, bracts and/or branches. Samples will not be collected to standardized or random plans rather samples will be collected solely from symptomatic plants. Representatives of some of the major seed companies will also be asked to contact the appropriate extension specialist when they receive calls from producers about bacterial blight fields. Additional isolates will be recovered from samples submitted for diagnosis by producers, consultants, crop advisors, extension agents or other representatives of agricultural industries. number of cases of bacterial blight which occur). The number of samples collected in a particular state or region will correspond with the availability of sampling sites (i.e. Sampling will be conducted following boll formation but prior to defoliation and harvest. Project Methods Survey: Field surveys will be conducted in Georgia, Mississippi and Texas. If a new race of Xcm is found, it will be made available to cotton breeding programs. Changes in production practices, particularly regarding cultivar choice, will be monitored by the extension specialists via surveys as well as general state-wide bacterial blight problems. Tests will be conducted in the greenhouse on varieties that should be resistant and susceptible to bacterial blight, using other bacteria that are isolated in close proximity to Xcm.Extension specialists in Georgia, Mississippi and Texas will develop material for producers to manage this disease using IPM principles including current cultivars and their susceptibility to the disease, importance of plowing in infected plant material, and planting seed that is clean of the bacteria. There is an additional hypothesis, that the resurgence of the disease is due to an interaction between Xcm and another bacteria. These tests should provide evidence if a race shift is occurring. In addition, Xcm isolates from Texas will be tested on cotton cultivars differing in their disease response to race 18 of Xcm. The Xcm isolates collected in this project will be characterized for T3Es. Most pathogenic Xanthomonads deploy 10-30 distinct T3E proteins during infection, and on a population level, these T3E repertoires are variable. Xcm injects type 3 effector proteins (T3Es) into plants to promote susceptibility in cultivars. The project proposes to collect a diverse set of Xcm from Georgia, Mississippi, and Texas. It must be controlled for sustainable production of cotton. malvacearum (Xcm), is a yield limiting disease of cotton and also survives and spreads in seed. ![]() Bacterial blight, caused by Xanthomonas citri subsp. Non Technical Summary This applied, research-led project addresses the resurgence of bacterial blight of cotton. ![]()
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